M-NUT/MAC Dipslides

USE
Isolation and differentiation of Gram (-) enteric bacilli. Coliform Testing / Recovering of Stressed Coliforms.

APPLICATION
In total coliform testing (TCC), the coliform organisms tested for include: total coliform, fecal coliform, and E. coli (Escherichia coli). Detection of fecal coliforms (a subset of total coliforms) or Escherichia coli (a subset of fecal coliforms) can indicate the potential presence of waterborne pathogens associated with fecal contamination.

PADDLE AGARS
Side 1: Nutrient-TTC Agar (NUT) ?Çô (Color: Yellow) General purpose (relatively non-selective) medium, which will support the growth of a wide variety of organisms. Suitable for cultivation of both aerobes and anaerobes. Aerobic coliform bacteria can be detected by their ability to reduce the TTC dye to a red-colored formozan dye. Bacterial colonies appear as red dots on an otherwise yellow medium.

Note: Paddle color is normally LIGHT YELLOW when the NUT agar is cast (about pH 6.0). Some microorganism growth (even before colonies are OBSERVABLE) will shift the pH from an acidic to a more alkaline level (pH 7.0 or higher) ?Çô turning the agar a light green.

Side 2: MacConkey Agar (MAC) ?Çô (Color: Watermelon) Both selective AND differential; used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms (Lac (+)) and lactose non-fermenters (Lac (-)), which include potential pathogens.
*Note: Side 1 of each paddle is marked with an indented laser line.